ZEISS ON YOUR CAMPUS

ZEISS Apotome Plus

Precision Redefined in Fluorescence Microscopy

Th 4, 05 tháng 3 2025 - Th 2, 02 tháng 6 2025

ZEISS On Your Campus (ZOYC) arrives in Malaysia. This event connects you with cutting-edge microscopy and live demonstrations. Learn how the ZEISS Apotome Plus transforms widefield microscopes to deliver confocal-like image quality.

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Confocal-like quality

Lateral resolution down to 180 nm for unmatched detail

Join us for an immersive hands-on workshop that brings you face-to-face with confocal-like image quality with your widefield microscope, where you can analyze your own samples and directly unleash the power of optical sectioning down to 180 nm.

This event is specifically designed for scientists who want to delve into the latest imaging techniques, enabling them to explore and optimize their own workflows with the support of experts from ZEISS Microscopy.

What Happens at ZOYC?

Seminar Day
Hear from ZEISS experts. Understand structured illumination and see how it removes out-of-focus light. Learn how to create optical sections, enhance resolution, and capture 3D images.

Hands-On Days
Image your own samples with ZEISS Apotome Plus. Work with advanced optical sectioning. Discover details hidden in thick specimens and complex samples.

Why Join?

Expand your understanding of fluorescence imaging.
Gain hands-on experience with Apotome Plus.
Take new insights into your research.

Who is it for?
You, if you work with microscopy in research, life sciences, or imaging.

What is ZEISS Apotome Plus?
Apotome Plus uses structured illumination to eliminate blur in fluorescence microscopy. It produces sharp, reliable optical sections and enhances 3D rendering. It resolves structures down to 180 nm with minimal effort.

Cos7 cells (nuclei stained with Hoechst, tubulin with Alexa 488 and Phalloidin with Alexa 568) imaged with Plan Apochromat 63x/1.4.

Reliable Optical Sections

Under Varying Experimental Conditions

Apotome 3 significantly increases the axial resolution compared to conventional fluorescence widefield microscopy: You obtain optical sections that allow 3D rendering, even from thick specimens. Three grids of different geometries give you the best resolution for each objective. You can focus on your experiment as the ideal illumination structure is automatically selected, always resulting in high-contrast optical sections.

_{Caption: Cos7 cells (nuclei stained with Hoechst, tubulin with Alexa 488 and Phalloidin with Alexa 568) imaged with Plan Apochromat 63x/1.4.}

Cortical neurons (left: Widefield; right: Apotome 3). Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.

Peer-reviewed Algorithms

Linear Approaches for True Optical Sections

Purely software-based methods require either prior knowledge of the sample (AI based methods) or rely on complex algorithms that have not been peer-reviewed. Users must trust that these black-box solutions do not falsify information when “enhancing” the image. ZEISS Apotome 3 uses the information from the structured illumination combined with documented algorithms to create a crisp optical section you can trust.

^{Caption: Cortical neurons (left: Widefield; right: Apotome 3). Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.}

35 μm sagittal section of adult mouse brain, imaged with ZEISS Axio Observer and ZEISS Apotome, processed with Apotome Plus. Sample courtesy of University of California, Davis / NIH NeuroMab Facility. — Sample courtesy of University of California, Davis / NIH NeuroMab Facility.

Sample courtesy of University of California, Davis / NIH NeuroMab Facility.

Confocal-like Image Quality

180 nm Resolution with Apotome Plus

Resolve details that were not visible before with your widefield microscope: With Apotome Plus, you can obtain structural information at lateral resolution down to 180 nm. The combination of structured illumination with state-of-the-art image processing substantially improves the signal-to-noise ratio and resolution in x, y, and z.

^{Caption: 35 μm sagittal section of adult mouse brain, imaged with ZEISS Axio Observer and ZEISS Apotome, processed with Apotome Plus. Sample courtesy of University of California, Davis / NIH NeuroMab Facility.}

Agenda

	Unlocking Precision in Fluorescence Microscopy with ZEISS Apotome Plus
09:00 – 09:30	Registration and Welcome Coffee Arrive, network, and collect materials.
09:30 – 09:45	Welcome Address Introduction to ZOYC and the importance of advancing microscopy techniques.
09:45 – 10:30	Keynote: Structured Illumination in Fluorescence Microscopy Understand the science behind structured illumination and how it achieves optical sectioning.
10:30 – 11:15	Session: ZEISS Apotome Plus – The System and Its Applications Learn how Apotome Plus works, its capabilities, and its relevance in biological research.
11:15 – 11:30	Break Refreshments and informal networking.
11:30 – 12:15	Case Studies: From Cells to Complex Samples Explore imaging examples, including live-cell imaging, thick tissue sections, and 3D renderings.
12:15 – 13:00	Live Demo: Imaging with ZEISS Apotome Plus See a real-time demonstration of optical sectioning and high-resolution imaging.
13:00 – 14:00	Lunch Break Enjoy lunch and connect with peers and ZEISS experts.
14:00 – 14:45	Interactive Workshop: Optimising Sample Preparation and Imaging Learn best practices for sample preparation and hands-on tips for optimal imaging.
14:45 – 15:30	Future Perspectives in Widefield Microscopy Discuss emerging trends and how ZEISS supports evolving research needs.
15:30 – 16:00	Q&A and Closing Remarks Ask questions and recap key takeaways.
16:00 – 17:00	Networking and Meet the Experts Engage directly with ZEISS specialists and explore additional resources.

	Unlocking Precision in Fluorescence Microscopy with ZEISS Apotome Plus
09:00 – 09:30	Registration and Welcome Coffee Arrive, network, and collect materials.
09:30 – 09:45	Welcome Address Introduction to ZOYC and the importance of advancing microscopy techniques.
09:45 – 10:30	Keynote: Structured Illumination in Fluorescence Microscopy Understand the science behind structured illumination and how it achieves optical sectioning.
10:30 – 11:15	Session: ZEISS Apotome Plus – The System and Its Applications Learn how Apotome Plus works, its capabilities, and its relevance in biological research.
11:15 – 11:30	Break Refreshments and informal networking.
11:30 – 12:15	Case Studies: From Cells to Complex Samples Explore imaging examples, including live-cell imaging, thick tissue sections, and 3D renderings.
12:15 – 13:00	Live Demo: Imaging with ZEISS Apotome Plus See a real-time demonstration of optical sectioning and high-resolution imaging.
13:00 – 14:00	Lunch Break Enjoy lunch and connect with peers and ZEISS experts.
14:00 – 14:45	Interactive Workshop: Optimising Sample Preparation and Imaging Learn best practices for sample preparation and hands-on tips for optimal imaging.
14:45 – 15:30	Future Perspectives in Widefield Microscopy Discuss emerging trends and how ZEISS supports evolving research needs.
15:30 – 16:00	Q&A and Closing Remarks Ask questions and recap key takeaways.
16:00 – 17:00	Networking and Meet the Experts Engage directly with ZEISS specialists and explore additional resources.

	Hands-on demonstration - Test your own specimen Register for an individual hands-on demonstration and test your own specimen using the Lattice SIM 3 or Celldiscoverer 7. Participation in the sample preparation presentation is strongly recommended for a successful demonstration.
Select your demonstration in the form	Gentle super-resolution live cell imaging with ZEISS Lattice SIM 3
	Adaptable automation for advanced workflows with ZEISS Celldiscoverer 7

Location

1. Universität Münster

Multiscale Imaging Centre Röntgenstr. 16 48149 Münster Germany

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Registration

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Please select your preferred date and system for the demonstration.
Registration includes one person, with the option to bring up to five additional members from your work group.